l? 2018-至今??? ?中科院廣州生物醫(yī)藥與健康研究院 研究員
l? 2016-2018?? ??UCB制藥/資深研究員
l? 2013-2016?? ??弗朗西斯克里克研究所/博士后 ?????
????????????????合作導(dǎo)師：John Diffley FRS? 英國皇家學(xué)會(huì)院士
l? 2012-2013?? ??癌癥研究所，倫敦大學(xué)/博士后?
????????????????合作導(dǎo)師：David Barford FRS 英國皇家學(xué)會(huì)院士????
l? 2008-2012??? ?癌癥研究所，倫敦大學(xué)/博士?????
????????????????指導(dǎo)導(dǎo)師：Edward Morris and David Barford
l? 2002-2006?? ??清華大學(xué)生物科學(xué)與技術(shù)系/學(xué)士?
????????????????指導(dǎo)導(dǎo)師:饒子和院士
真核生物染色質(zhì)的結(jié)構(gòu)與功能受到眾多表觀調(diào)控因子在時(shí)間與空間水平上的精準(zhǔn)調(diào)控，從而確保生命活動(dòng)的有序進(jìn)行。DNA組蛋白和核小體的分子結(jié)構(gòu)與組成形式是造成染色質(zhì)動(dòng)態(tài)結(jié)構(gòu)變化的基礎(chǔ)，并與其他表觀因子如組蛋白變體、組蛋白伴侶、組蛋白修飾酶和染色質(zhì)重塑復(fù)合物等一起調(diào)控染色質(zhì)的動(dòng)態(tài)變化和功能。針對這一重要生命過程，課題組主要從以下幾個(gè)方向展開研究工作：
1. 重要表觀遺傳調(diào)控相關(guān)復(fù)合體的功能和分子機(jī)制
染色質(zhì)的表觀遺傳調(diào)控由相應(yīng)的大分子復(fù)合物來完成，想要充分闡明表觀遺傳信息是如何被呈現(xiàn)和維持的，就必須對這些調(diào)控復(fù)合物有分子水平上的理解。課題組通過生物化學(xué)和冷凍電鏡等手段，對乙?；头核鼗嚓P(guān)的表觀復(fù)合物開展功能和結(jié)構(gòu)研究，揭示其發(fā)揮生理功能的分子構(gòu)架特征。這些復(fù)合物往往與腫瘤等重要疾病發(fā)生密切相關(guān)，我們希望能進(jìn)一步在分子水平上理解其參與腫瘤發(fā)生發(fā)展的致病機(jī)理，并以此鑒定出創(chuàng)新性藥物靶標(biāo)蛋白。
2.染色質(zhì)動(dòng)態(tài)的原位研究
常染色質(zhì)和異染色質(zhì)的動(dòng)態(tài)變化是細(xì)胞命運(yùn)轉(zhuǎn)換的關(guān)鍵，但是這一變化的具體分子過程和調(diào)控機(jī)制還有很多未知之處。課題組致力于在細(xì)胞核內(nèi)原位環(huán)境中，在傳統(tǒng)細(xì)胞生物學(xué)研究的基礎(chǔ)上，結(jié)合轉(zhuǎn)錄組、蛋白質(zhì)組等組學(xué)手段和超分辨結(jié)構(gòu)光成像、冷凍電子斷層成像等成像手段，全面揭示多種表觀因子與染色質(zhì)的相互作用網(wǎng)絡(luò)，從而在多尺度上描繪這些表觀因子所主導(dǎo)的染色質(zhì)動(dòng)態(tài)變化的分子景觀藍(lán)圖。
3. 基于人工智能的冷凍電鏡研究
課題組致力于通過AI算法的開發(fā)與應(yīng)用，一方面旨在提升Cryo-EM實(shí)驗(yàn)流程的自動(dòng)化程度和數(shù)據(jù)收集效率；另一方面，則聚焦于對海量成像數(shù)據(jù)進(jìn)行深度挖掘與分析，以期揭示更為精細(xì)、全面的生物分子結(jié)構(gòu)與功能信息，從而為表觀遺傳調(diào)控及染色質(zhì)動(dòng)態(tài)學(xué)的深入研究提供強(qiáng)有力的技術(shù)支持與數(shù)據(jù)洞察。
主要學(xué)術(shù)成果：
何俊博士，現(xiàn)任中國科學(xué)院廣州生物醫(yī)藥與健康研究院研究員、博士生導(dǎo)師，入選中科院高層次人才項(xiàng)目，并兼任中國生物物理協(xié)會(huì)冷凍電子顯微學(xué)分會(huì)委員、中國電子顯微鏡學(xué)會(huì)廣東省分會(huì)委員。主要專注于利用冷凍電鏡等手段研究生物大分子的高分辨率三維結(jié)構(gòu)特征，探索在生理和病理過程中起到重要作用的表觀遺傳調(diào)控機(jī)器的結(jié)構(gòu)和分子機(jī)理。以第一作者和通訊作者身份在Cell Research、Molecular Cell、Nature Microbiology和 Nature Communication等國際高影響期刊上發(fā)表論文數(shù)十篇，多次受邀在國內(nèi)外會(huì)議上作學(xué)術(shù)報(bào)告。同時(shí)承擔(dān)國家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目、國家自然科學(xué)基金國際(地區(qū))合作與交流項(xiàng)目、國家自然科學(xué)基金專項(xiàng)項(xiàng)目、國家自然科學(xué)基金面上項(xiàng)目、廣東省自然科學(xué)基金面上項(xiàng)目等多個(gè)科研任務(wù)。
中國生物物理學(xué)會(huì)冷凍電子顯微學(xué)分會(huì) 委員
中國電子顯微鏡學(xué)會(huì)廣東省分會(huì) 委員中國生物物理學(xué)會(huì)冷凍電子顯微學(xué)分會(huì) 理事
2013 Chairman’s Prize for Best PhD, the Institute of Cancer Research
2012 Chinese Government Award for outstanding students abroad
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發(fā)明專利：
通過設(shè)計(jì)和改造二硫鍵交聯(lián)位點(diǎn)的SLC膜蛋白復(fù)合物的制備方法 何俊，官嘉麗，鄒彬倩 （專利號：ZL 2023 1 1158832.1）
軟件著作：
冷凍電鏡前期數(shù)據(jù)質(zhì)控軟件（cryoPurifier） （軟件著作登記號：2024SR0491806 ）
學(xué)生獲獎(jiǎng)情況：
2021級
董淑琦（博士生） 榮獲中國科學(xué)院院長獎(jiǎng)優(yōu)秀獎(jiǎng)（2024年）、國家獎(jiǎng)學(xué)金（2023年）、中國科學(xué)院大學(xué)優(yōu)秀畢業(yè)生（2024年）、北京市優(yōu)秀畢業(yè)生（2024年）、三好學(xué)生（2023年）、GIBH優(yōu)秀研究生獎(jiǎng)學(xué)金一等獎(jiǎng)（2024年）
2022級
官嘉麗（博士生）? 榮獲三好學(xué)生（2024年）、三等獎(jiǎng)學(xué)金（2024年）
趙河豫（博士生）? 榮獲三好學(xué)生（2024年）
蘇小鳳（碩士生）? 榮獲三好學(xué)生（2024年）
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