1984年在四川畜牧獸醫(yī)學(xué)院獲學(xué)士學(xué)位，1987年在中國(guó)人民解放軍獸醫(yī)大學(xué)獲碩士學(xué)位，1995年在東北農(nóng)業(yè)大學(xué)獲博士學(xué)位。1995年至1997年在中國(guó)人民解放軍農(nóng)牧大學(xué)從事博士后研究。1998年赴美國(guó)密蘇里大學(xué)動(dòng)物研究中心從事博士后研究，2002年晉升為美國(guó)密蘇里大學(xué)動(dòng)物研究中心研究助理教授。2007年7月回國(guó)，擔(dān)任中國(guó)科學(xué)院廣州生物醫(yī)藥與健康研究院研究員、博士生導(dǎo)師。

1.大動(dòng)物干細(xì)胞：大動(dòng)物多能干細(xì)胞分離培養(yǎng)體系的建立與寵物干細(xì)胞治療的有效性及安全性研究；

2.大動(dòng)物克?。涸谔剿黧w細(xì)胞重編程機(jī)制的基礎(chǔ)上，優(yōu)化大動(dòng)物體細(xì)胞克隆技術(shù)，提高體細(xì)胞重編程效率，開(kāi)展豬、犬、貓以及家兔等大動(dòng)物的克隆。

3.新型基因修飾技術(shù)開(kāi)發(fā)與優(yōu)化：建立精準(zhǔn)、高效和安全的大動(dòng)物基因編輯技術(shù)體系。

4. 基因修飾大動(dòng)物的培育：將體細(xì)胞克隆技術(shù)與基因修飾技術(shù)相結(jié)合，培育在農(nóng)業(yè)和生物醫(yī)藥有重要價(jià)值的基因修飾大動(dòng)物模型。

5.人源化基因修飾大動(dòng)物模型的轉(zhuǎn)化應(yīng)用：利用含有人源化功能蛋白分子基因修飾豬制備可用于人類疾病治療的生物活性制品；利用免疫原性人源化的豬器官開(kāi)展異種器官移植的臨床前研究；利用人源化基因修飾大動(dòng)物疾病模型開(kāi)展基因治療、干細(xì)胞治療以及創(chuàng)新藥物治療的有效性與安全性評(píng)估。

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1.國(guó)家自然科學(xué)基金區(qū)域創(chuàng)新發(fā)展聯(lián)合基金重點(diǎn)支持項(xiàng)目（2024/01-2027/12），項(xiàng)目負(fù)責(zé)人；

2.國(guó)家重點(diǎn)研發(fā)計(jì)劃“干細(xì)胞研究與器官修復(fù)”重點(diǎn)專項(xiàng)項(xiàng)目（2022/12-2027/11），項(xiàng)目負(fù)責(zé)人；

3.中國(guó)科學(xué)院“器官重建與制造”戰(zhàn)略性先導(dǎo)科技專項(xiàng)（A類）（2017/12-2022/11），課題負(fù)責(zé)人；

4.國(guó)家重點(diǎn)研發(fā)計(jì)劃“干細(xì)胞及轉(zhuǎn)化研究”重點(diǎn)專項(xiàng)項(xiàng)目（2017/07-2022/06），項(xiàng)目負(fù)責(zé)人；

5.國(guó)家重大科學(xué)研究計(jì)劃（973）項(xiàng)目（2011/01-2015/08），項(xiàng)目負(fù)責(zé)人；

6.國(guó)家高技術(shù)研究發(fā)展計(jì)劃（863）重點(diǎn)項(xiàng)目（2006/12-2010/12），項(xiàng)目負(fù)責(zé)人。

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1.?Weikai Ge^#, Shixue Gou^#, Xiaozhu Zhao^#, Qin Jin^#, Zhenpeng Zhuang, Yu Zhao, Yanhui Liang, Zhen Ouyang, Xiaoyi Liu, Fangbing Chen, Hui Shi, Haizhao Yan, Han Wu*, Liangxue Lai*, Kepin Wang*, In vivo evaluation of guide-free Cas9-induced safety risks in a pig model, Signal Transduction and Targeted Therapy, 2024. Accepted.

2.?Jiaowei Wang^#, Wenguang Xie^#, Nan Li^#, Wenjuan Li^#, Zhishuai Zhang^#, Nana Fan, Zhen Ouyang, Yu Zhao, Chengdan Lai, Hao Li, Mengqi Chen, Longquan Quan, Yunpan Li, Yu Jiang, Wenqi Jia, Lixin Fu, Md Abdul Mazid, Yanling Zhu, Patrick H Maxwell, Guangjin Pan*, Miguel A Esteban*, Zhen Dai*, Liangxue Lai*, Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation, Cell Stem Cell, 2023, 30(9):1235-1245.

3.?Sen Yan^#*, Xiao Zheng, Yingqi Lin, Caijuan Li, Zhaoming Liu, Jiawei Li, Zhuchi Tu, Yu Zhao, Chunhui Huang, Yizhi Chen, Jun Li, Xichen Song, Bofeng Han, Wei Wang, Weien Liang, Liangxue Lai*, Xiao-Jiang Li*, Shihua Li*, Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington's disease, Nature Biomedical Engineering, 2023, 7(5):629-646.

4.?Qin Jin^#, Xiaoyi Liu^#, Zhenpeng Zhuang^#, Jiayuan Huang^#, Shixue Gou, Hui Shi, Yu Zhao, Zhen Ouyang, Zhaoming Liu, Lei Li, Junjie Mao, Weikai Ge, Fangbing Chen, Manya Yu, Yezhi Guan, Yinghua Ye, Chengcheng Tang, Ren Huang, Kepin Wang*, Liangxue Lai*, Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation, Genome Biology, 2023, 24:8.

5.?Qin Jin^#, Xiaoyu Yang, Shixue Gou, Xiaoyi Liu, Zhenpeng Zhuang, Yanhui Liang, Hui Shi, Jiayuan Huang, Han Wu, Yu Zhao, Zhen Ouyang, Quanjun Zhang, Zhaoming Liu, Fangbing Chen, Weikai Ge, Jingke Xie, Nan Li, Chengdan Lai, Xiaozhu Zhao, Jiaowei Wang, Meng Lian, Lei Li, Longquan Quan, Yinghua Ye, Liangxue Lai*, Kepin Wang*, Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression, Science China Life Sciences, 2022, 65(11):2269-2286.

6.?Yanhui Liang^#, Jingke Xie^#, Quanjun Zhang^#, Xiaomin Wang^#, Shixue Gou, Lihui Lin, Tao Chen, Weikai Ge, Zhenpeng Zhuang, Meng Lian, Fangbing Chen, Nan Li, Zhen Ouyang, Chengdan Lai, Xiaoyi Liu, Lei Li, Yinghua Ye, Han Wu, Kepin Wang*, Liangxue Lai*, AGBE: A dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns, Nucleic Acids Research, 2022, 50(9):5384-5399.

7.?Xiaomin Wang^#, Yanhui Liang^#, Jianping Zhao^#, Yuan Li, Shixue Gou, Min Zheng, Juanjuan Zhou, Quanjun Zhang, Jidong Mi*, Liangxue Lai*, Generation of permanent neonatal diabetes mellitus dogs with glucokinase point mutations through base editing, Cell Discovery, 2021, 7:92.

8.?Jingke Xie^#, Weikai Ge^#, Nan Li^#, Qishuai Liu^#, Fangbing Chen, Xiaoyu Yang, Xingyun Huang, Zhen Ouyang, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Shixue Gou, Han Wu, Chengdan Lai, Nana Fan, Qin Jin, Hui Shi, Yanhui Liang, Ting Lan, Longquan Quan, Xiaoping Li, Kepin Wang*, Liangxue Lai*, Efficient base editing for multiple genes/loci in pigs using base editors, Nature Communications, 2019, 10:2852.

9.?Sen Yan^#, Zhuchi Tu^#, Zhaoming Liu^#, Nana Fan, Huiming Yang, Su Yang, Weili Yang, Yu Zhao, Zhen Ouyang, Chengdan Lai, Huaqiang Yang, Li Li, Qishuai Liu, Hui Shi,2 Guangqing Xu, Heng Zhao, Hongjiang Wei, Zhong Pei, Shihua Li*, Liangxue Lai*, Xiao-Jiang Li*, A huntingtin knock-in pig model recapitulates features of selective neurodegeneration in Huntington’s disease, Cell, 2018, 173(4):989-1002.

10.?Kepin Wang^#, Qin Jin^#, Degong Ruan^#, Yi Yang, Qishuai Liu, Han Wu, Zhiwei Zhou, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Jiangyun Peng, Chengdan Lai, Nana Fan, Yanhui Liang, Ting Lan, Nan Li, Xiaoshan Wang, Xinlu Wang, Yong Fan, Pieter A. Doevendans, Joost P.G. Sluijter, Pentao Liu, Xiaoping Li*, Liangxue Lai*, Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing, Genome Research, 2017, 27(12): 2061-2071.

11.Yi Yang^#, Kepin Wang^#, Han Wu, Qin Jin, Degong Ruan, Zhen Ouyang, Bentian Zhao, Zhaoming Liu, Yu Zhao, Quanjun Zhang, Nana Fan, Qishuai Liu, Shimei Guo, Lei Bu, Yong Fan, Xiaofang Sun, Xiaoping Li*, Liangxue Lai*, Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering, Journal of Molecular Cell Biology, 2016, 8(2): 174-177.

12.?Guohao Wang^#, Huaqiang Yang^#, Sen Yan^#, Chuan-En Wang, Xudong Liu, Bentian Zhao, Zhen Ouyang, Peng Yin, Zhaoming Liu, Yu Zhao, Tao Liu, Nana Fan, Lin Guo, Shihua Li, Xiao-Jiang Li*, Liangxue Lai*, Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain, Molecular Neurodegeneration, 2015, 10:42.

13.?Huaqiang Yang^#, Guohao Wang^#, Haitao Sun^#, Runzhe Shu^#, Tao Liu, Chuan-En Wang, Zhaoming Liu, Yu Zhao, Bentian Zhao, Zhen Ouyang, Dongshan Yang, Jiao Huang, Yueling Zhou, Shihua Li, Xiaodan Jiang*, Zhicheng Xiao*, Xiao-Jiang Li*, Liangxue Lai*, Species-dependent neuropathology in transgenic SOD1 pigs, Cell Research, 2014, 24(4):464-481.

14.?Xiaoping Li^#, Yi Yang^#, Lei Bu, Xiaogang Guo, Chengcheng Tang, Jun Song, Nana Fan, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Xiaoling Yi, Longquan Quan, Songcai Liu, Zhenguo Yang, Hongsheng Ouyang, Y Eugene Chen, Zhong Wang*, Liangxue Lai*, Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing, Cell Research, 2014, 24(4):501-504.

15.?Jun Song, Juan Zhong, Xiaogang Guo, Yongqiang Chen, Qingjian Zou, Jiao Huang, Xiaoping Li, Quanjun Zhang, Zhiwu Jiang, Chengcheng Tang, Huaqiang Yang, Tao Liu, Peng Li, Duanqing Pei, Liangxue Lai, Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs, Cell Research, 2013, 23(8):1059-1062.

16.?Nana Fan^#, Jijun Chen^#, Zhouchun Shang^#, Hongwei Dou, Guangzhen Ji, Qingjian Zou, Lu Wu, Lixiazi He, Fang Wang, Kai Liu, Na Liu, Jianyong Han, Qi Zhou, Dengke Pan, Dongshan Yang, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Lin Lin, Chongming Zhong, Quanlei Wang, Shouqi Wang, Ying Xu, Jing Luan, Yu Liang, Zhenzhen Yang, Jing Li, Chunxia Lu, Gábor Vajta, Ziyi Li, Hongsheng Ouyang, Huayan Wang, Yong Wang, Yang Yang, Zhonghua Liu, Hong Wei, Zhidong Luan, Miguel A Esteban, Hongkui Deng, Huanming Yang, Duanqing Pei, Ning Li, Gang Pei, Lin Liu, Yutao Du*, Lei Xiao*, Liangxue Lai*, Piglets cloned from induced pluripotent stem cells, Cell Research, 2013, 23(1):162-166.

17.?Dongshan Yang^#, Huaqiang Yang^#, Wei Li, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Nana Fan, Jun Song, Jiangtian Tian, Feng Li, Jifeng Zhang, Lin Chang, Duanqing Pei, Y Eugene Chen*, Liangxue Lai*, Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning, Cell Research, 2011, 21(6):979-982.

18.?Liangxue Lai^#, Jing X Kang^#, Rongfeng Li, Jingdong Wang, William T Witt, Hwan Yul Yong, Yanhong Hao, David M Wax, Clifton N Murphy, August Rieke, Melissa Samuel, Michael L Linville, Scott W Korte, Rhobert W Evans, Thomas E Starzl, Randall S Prather*, Yifan Dai*, Generation of cloned transgenic pigs rich in omega-3 fatty acids, Nature Biotechnology, 2006, 24(4):435-436.

19.?Donna Kolber-Simonds^#, Liangxue Lai^#, Steven R Watt^#, Maria Denaro^#, Scott Arn, Monica L Augenstein, Jeffery Betthauser, David B Carter, Julia L Greenstein, Yanhong Hao, Gi-Sun Im, Zhonghua Liu, Greg D Mell, Clifton N Murphy, Kwang-Wook Park, August Rieke, David J J Ryan, David H Sachs, Erik J Forsberg, Randall S Prather, Robert J Hawley*, Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations, Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(19):7335-7340.

20.?Liangxue Lai^#, Donna Kolber-Simonds, Kwang-Wook Park, Hee-Tae Cheong, Julia L Greenstein, Gi-Sun Im, Melissa Samuel, Aaron Bonk, August Rieke, Billy N Day, Clifton N Murphy, David B Carter, Robert J Hawley, Randall S Prather*, Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning, Science, 2002, 295(5557):1089-1092.

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